HPLC optimization using Luna Omega 5um Polar C18 150x4.6mm

LC Conditions

Column

Luna Omega 5 µm Polar C18 100Å, LC Column 150 x 4.6 mm, EA

Brand

Luna Omega

Part No

00F-4754-E0

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% TFA

B: Acetonitrile with 0.1% TFA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

15

10

90

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info