FAMEs In Infant Formula by GC/FID on Zebron ZB-FAME

GC Conditions

Column

Zebron ZB-FAME, GC Cap. Column 30m x 0.25mm x 0.2 µm, Ea

Part No

7HG-G033-10

Phase Name

No Phase

Injection

Split 50:1 @ 240°C

Oven Program

100 ºC for 2 min to 140 ºC @ 10 °C/min to 190 ºC @ 3 ºC/min to 260 ºC @ 30 ºC/min for 2 min

Carrier Gas

Constant Flow Helium, 1.2 mL/min

Detection

Flame Ionization (FID) @ (260°C)

Detection Info

Sample Notes

Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID Liner Part No.: AG2-0A11-05 (for Agilent systems) Inlet Seal: AG0-8620 (Gold Plated Easy Seal) Septum: AG0-4696 (PhenoRed-400) Sample Preparation: 1. Weigh out 500 mg of powdered infant formula into a scintillation vial 2. Dissolve (or dilute) sample in 5 mL toluene 3. Add 6 mL 10 % acetyl chloride solution in methanol 4. Incubate @ 80 °C for 2 hours 5. After incubation add 10 mL 10 % Na2CO3 solution and centrifuge at 5000 rpm for 5 min 6. Extract organic layer for GC analysis