Underivatized MMA and Succinic Acid from Plasma on Luna Omega 1.6u PS C18, 50x2.1mm with Strata-X-AW

LC Conditions

Column

Luna Omega 1.6µm PS C18 100Å, LC Column 50 x 2.1 mm, EA

Brand

Luna Omega

Part No

00B-4752-AN

Phase Name

PS C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in H2O

B: 0.1% Formic Acid in ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

2

10

90

3

3

10

90

4

3.01

98

2

5

5

98

2

Flow Rate

0.40 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (600°C)

Detection Info

Sample Notes

MMA (117.0-->72.9; 117.0-->54.9) MMA-D3 (120.1-->75.9)

Sample Preparation Details

Description

Strata-X-AW 33 µm Polymeric Weak Anion, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S038-TAK

Condition 1

1 mL Methanol

Condition 2

1 mL 0.1% Acetic Acid in Water

Load

Load 100.00 µL Sample diluted in 0.1% Acetic Acid in Water

Wash 1

0.5 mL 50:50 Methanol:Water

Dry

5-10 min under high vacuum

Elution 1

0.6 mL 2% Ammonium Hydroxide in Methanol

Elution 2

0.6 mL 2% Ammonium Hydroxide in Methanol

Evaporation

Evaporate to dryness

Reconstitute

200 µL Mobile Phase A