FAMEs In Extra Virgin Olive Oil by GC/FID on Zebron ZB-FAME (30 m)

GC Conditions

Column

Zebron ZB-FAME, GC Cap. Column 30m x 0.25mm x 0.2 µm, Ea

Part No

7HG-G033-10

Phase Name

No Phase

Injection

Split 50:1 @ 240°C

Oven Program

100 °C for 2 min to 140 °C @ 10 °C/min to 190 °C @ 3 °C/min to 260 °C @ 30 °C/min for 2 min

Carrier Gas

Constant Flow Helium, 1.2 mL/min

Detection

Flame Ionization (FID) @ (260°C)

Detection Info

Sample Notes

Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID Liner Part No.: AG2-0A11-05 (for Agilent systems) Inlet Seal: AG0-8620 (Gold Plated Easy Seal) Septum: AG0-4696 (PhenoRed-400) Sample Preparation: 1. Strata® Si-1 Tube, 1 g/6 mL (Part No.: 8B-S012-JCH) on a vacuum or positive pressure manifold 2. Wash cartridge with 6 mL of hexane 3. Load oil solution (0.12 g of oil in 0.5 mL of hexane) 4. Elute with 10 mL of hexane/diethyl ether (87:13) 5. Evaporate eluate under a steady stream of nitrogen 6. Dissolve purified oil residue in 1mL of heptane 7. Add 0.1 mL of 2N potassium hydroxide in methanol to purified oil solution 8. Cap tube and shake vigorously for 15 seconds 9. Leave to separate until upper layer becomes clear 10. Extract upper layer for GC analysis

Sample Preparation Details

Pretreatment Note

To reconstituted sample, add 0.1 mL of 2 N Potassium hydroxide in methanol. Cap tube and shake vigorously for 15 seconds. Leave to separate until upper layer becomes clear. Extract upper layer for GC anlaysis.

Condition 1

6 mL Hexane

Load

Load 0.50 mL Sample diluted in Pretreated sample

Elution 1

10 mL Hexane/Diethyl ether (87:13)

Evaporation

Evaporate to dryness

Reconstitute

1 mL Heptane