Barbiturates from urine by Strata X (60mg/3cc) (TIC)

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 280°C

Oven Program

145 °C to 300 °C @ 25 °C/min

Carrier Gas

Constant Flow Helium, 0.6 mL/min

Detection

Mass Selective (MSD) @ (320°C)

Detection Info

Sample Notes

Analytes are 300ng/mL in urine derivatized with TMPAH Sim ions: (Quant ion*) D5-Butalbital (*201,184,214): Butalbital (*196,181,138): Amobarbital (*169,184,225): Pentobarbital (*169,184,225): Secobarbital (*196,181,138): D5-Phenobarbital (*233,151,122): Phenobarbital (*232,146,117)

Sample Preparation Details

Pretreatment Note

Reconstitute with 100uL Acetonitrile and 50uL of Thermo Scientific MethElute Reagent (TS49301)

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

3 mL Water at 1 mL/min

Condition 3

1 mL 20mM potassium phosphate pH 2.7 at 1 mL/min

Load

Load 2.00 mL Sample diluted in Diluted Urine

Wash 1

3 mL Water at 1 mL/min

Wash 2

3 mL 100mM acetic acid at 1 mL/min

Dry

Dry column (5 minutes at > 10 inches Hg)

Elution 1

3 mL 85:15 Ethyl Acetate:Isopropyl Alcholol at 0.5 mL/min

Evaporation

Evaporate to dryness

Reconstitute

150 µL Acetonitrile/Thermo Scientific MethElute Reagent