Cyclosporine USP on Kinetex 2.6u Polar C18 50x2.1mm

LC Conditions

Column

Kinetex 2.6 µm Polar C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4759-AN

Phase Name

Polar C18

Elution Type

Isocratic

Mobile Phase

A: Acetonitrile, tert-butyl methyl ether, water, and phosphoric acid (430:50:520:1)

Gradient Profile

Step No.

Time(min)

%A

%B

1

30

100

0

Flow Rate

0.30 mL/min

Temperature

80°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info