FAMEs In Peanut Oil by GC/FID on Zebron ZB-FAME

GC Conditions

Column

Zebron ZB-FAME, GC Cap. Column 60m x 0.25mm x 0.2 µm, Ea

Part No

7KG-G033-10

Phase Name

ZB-FAME

Injection

Split 100:1 @ 240°C

Oven Program

180 °C isothermal

Carrier Gas

Constant Flow Helium, 1.2 mL/min

Detection

Flame Ionization (FID) @ (240°C)

Detection Info

Sample Notes

Recommended Accessories: Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID Liner Part No.: AG2-0A11-05 (for Agilent systems) Inlet Seal: AG0-8620 (Gold Plated Easy Seal) Septum: AG0-4696 (PhenoRed-400) Sample Preparation: 1. Weigh 0.2 g of sample into a glass test tube 2. Add 3.8 mL of hexane and vortex 3. Condition SPE tube (Strata® Si-1, 2 g/12 mL, Part No.: 8B-S012-KDG) under vacuum with 6 mL of hexane; do not let bed go dry 4. Load sample with light vacuum 5. Elute with 5 mL of hexane:ethyl acetate (87:13) 6. Evaporate under nitrogen at 35 °C 7. Reconstitute with 4 mL of hexane 8. Add 200 µL of 2 M potassium hydroxide/methanol solution and vortex 9. Leave at room temperature for 5 minutes 10. Add 2 mL of deionized water and vortex 11. Leave to separate until upper layer becomes clear 12. Extract upper layer for GC analysis