Apomyoglobin mapping using Luna Omega 1.6um C18 for Selectivity

LC Conditions

Column

Luna Omega 1.6µm C18 100Å, LC Column 50 x 2.1 mm, EA

Brand

Luna Omega

Part No

00B-4742-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% formic acid

B: Acetonitrile with 0.1% formci acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

30

50

50

Flow Rate

0.50 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info