uCATs by LCMS using Luna Omega 3um Polar C18 50x4.6mm

uCATs by LCMS using Luna Omega 3um Polar C18 50x4.6mm
LC Conditions
Column

Luna Omega 3 µm Polar C18 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00B-4760-E0

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% formic acid

B: Methanol with 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

3

0

100

Flow Rate

0.70 mL/min

Temperature

25°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info
Sample Preparation Details
Description

Strata-X-CW Polymeric Weak Cation, microelution 96-well plate, 2mg/well, EA

Part No

8M-S035-4GA

Pretreatment Note

Pool eluents and dilute with 100 µL of 0.1% Formic acid in water

Condition 1

0.2 mL Methanol

Condition 2

0.2 mL 50 mM Ammonium acetate, pH 7

Load

Load 1.00 mL Sample diluted in Pretreated sample

Wash 1

0.2 mL 50 mM Ammonium acetate, pH 7

Wash 2

0.2 mL Acetonitrile / Isopropyl acohol (1:1)

Dry

3 minutes under high vacuum

Elution 1

0.025 mL Water / Acetonitrile / Formic acid (85:10:5)

Elution 2

0.025 mL Water / Acetonitrile / Formic acid (85:10:5)

Evaporation

Evaporate to dryness

Order Items Used in This Application

Luna Omega 3 µm Polar C18 100 Å, LC Column 50 x 4.6 mm, Ea
00B-4760-E0

Luna Omega 3 µm Polar C18 100 Å, LC Column 50 x 4.6 mm, Ea

View Product

Strata-X-CW Polymeric Weak Cation, microelution 96-well plate, 2mg/well, EA
8M-S035-4GA

Strata-X-CW Polymeric Weak Cation, microelution 96-well plate, 2mg/well, EA

View Product

  • 1. Metanephrine
  • 2. Normetanephrine
  • 3. 3-Methoxytyramine
-OEChem-06211003162D<br><br> 29 29  0     1  0  0  0  0  0999 V2000<br>    3.7320    1.6550    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.8660   -1.8450    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.5981   -2.8450    0.0000 O   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4641    2.6550    0.0000 N   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.5981    1.1550    0.0000 C   0  0  3  0  0  0  0  0  0  0  0  0<br>    4.5981    0.1550    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4641    1.6550    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320   -0.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4641   -0.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320   -1.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.4641   -1.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.5981   -1.8450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.3301    3.1550    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.0000   -1.3450    0.0000 C   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.5981    2.0050    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.6762    1.0724    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.0747    1.7627    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.1951   -0.0350    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.0010   -0.0350    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    4.9272    2.9650    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.0010   -1.6550    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    3.7320    2.2750    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.6401    2.6181    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.8671    3.4650    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    6.0201    3.6919    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    5.1350   -3.1550    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    2.3100   -0.8081    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.4631   -1.0350    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>    1.6900   -1.8819    0.0000 H   0  0  0  0  0  0  0  0  0  0  0  0<br>  1  5  1  0  0  0  0<br>  1 22  1  0  0  0  0<br>  2 10  1  0  0  0  0<br>  2 14  1  0  0  0  0<br>  3 12  1  0  0  0  0<br>  3 26  1  0  0  0  0<br>  4  7  1  0  0  0  0<br>  4 13  1  0  0  0  0<br>  4 20  1  0  0  0  0<br>  5  6  1  0  0  0  0<br>  5  7  1  0  0  0  0<br>  5 15  1  0  0  0  0<br>  6  8  2  0  0  0  0<br>  6  9  1  0  0  0  0<br>  7 16  1  0  0  0  0<br>  7 17  1  0  0  0  0<br>  8 10  1  0  0  0  0<br>  8 18  1  0  0  0  0<br>  9 11  2  0  0  0  0<br>  9 19  1  0  0  0  0<br> 10 12  2  0  0  0  0<br> 11 12  1  0  0  0  0<br> 11 21  1  0  0  0  0<br> 13 23  1  0  0  0  0<br> 13 24  1  0  0  0  0<br> 13 25  1  0  0  0  0<br> 14 27  1  0  0  0  0<br> 14 28  1  0  0  0  0<br> 14 29  1  0  0  0  0<br>M  END
Compound Name

Metanephrine

CID

21100

Molecular Formula

C10H15NO3

Molecular Weight

197

No. Hydrogen Bond Acceptors

4

No. Hydrogen Bond Donors

3

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