uCATs by LCMS using Kinetex 2.6um Polar C18 50x4.6mm
LC Conditions
Column
Kinetex 2.6 µm Polar C18 100 Å, LC Column 50 x 4.6 mm, Ea
Brand
Kinetex
Part No
00B-4759-E0
Phase Name
Polar C18
Elution Type
Gradient
Mobile Phase
A: Water with 0.1% formic acid
B: Methanol with 0.1% formic acid
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
100
0
2
3
0
100
Flow Rate
0.70 mL/min
Temperature
25°C
Detection
Tandem Mass Spec (MS-MS) @ (22°C)
Detection Info
Sample Preparation Details
Description
Strata-X-CW Polymeric Weak Cation, microelution 96-well plate, 2mg/well, EA
Part No
8M-S035-4GA
Pretreatment Note
Pool eluents and dilute with 100 µL of 0.1% Formic acid in water
Condition 1
0.2 mL Methanol
Condition 2
0.2 mL 50 mM Ammonium acetate, pH 7
Load
Load 1.00 mL Sample diluted in Pretreated sample
Wash 1
0.2 mL 50 mM Ammonium acetate, pH 7
Wash 2
0.2 mL Acetonitrile/ Isopropyl alcohol (1:1)
Dry
3 minutes under high vacuum
Elution 1
0.025 mL Water / Acetonitrile / Formic acid (85:10:5)
Elution 2
0.025 mL Water / Acetonitrile / Formic acid (85:10:5)
Evaporation
Evaporate to dryness
Order Items Used in This Application
8M-S035-4GA
Strata-X-CW Polymeric Weak Cation, microelution 96-well plate, 2mg/well, EA
- 1. Metanephrine
- 2. Normetanephrine
- 3. 3-Methoxytyramine
-OEChem-06211003162D<br><br> 29 29 0 1 0 0 0 0 0999 V2000<br> 3.7320 1.6550 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -1.8450 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -2.8450 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 2.6550 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 1.1550 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 4.5981 0.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 1.6550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -0.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 -0.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -1.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 -1.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -1.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3301 3.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -1.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 2.0050 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6762 1.0724 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0747 1.7627 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1951 -0.0350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 -0.0350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.9272 2.9650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0010 -1.6550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 2.2750 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.6401 2.6181 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8671 3.4650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0201 3.6919 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -3.1550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3100 -0.8081 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4631 -1.0350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.6900 -1.8819 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 5 1 0 0 0 0<br> 1 22 1 0 0 0 0<br> 2 10 1 0 0 0 0<br> 2 14 1 0 0 0 0<br> 3 12 1 0 0 0 0<br> 3 26 1 0 0 0 0<br> 4 7 1 0 0 0 0<br> 4 13 1 0 0 0 0<br> 4 20 1 0 0 0 0<br> 5 6 1 0 0 0 0<br> 5 7 1 0 0 0 0<br> 5 15 1 0 0 0 0<br> 6 8 2 0 0 0 0<br> 6 9 1 0 0 0 0<br> 7 16 1 0 0 0 0<br> 7 17 1 0 0 0 0<br> 8 10 1 0 0 0 0<br> 8 18 1 0 0 0 0<br> 9 11 2 0 0 0 0<br> 9 19 1 0 0 0 0<br> 10 12 2 0 0 0 0<br> 11 12 1 0 0 0 0<br> 11 21 1 0 0 0 0<br> 13 23 1 0 0 0 0<br> 13 24 1 0 0 0 0<br> 13 25 1 0 0 0 0<br> 14 27 1 0 0 0 0<br> 14 28 1 0 0 0 0<br> 14 29 1 0 0 0 0<br>M END
Compound Name
Metanephrine
CID
21100
Molecular Formula
C10H15NO3
Molecular Weight
197
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3
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