Chromatographic Separation of Isomeric GCDCA and GDCA on Kinetex 2.6µm Polar C18 100x2.1 Column

LC Conditions

Column

Kinetex 2.6 µm Polar C18 100 Å, LC Column 100 x 2.1 mm, Ea

Brand

Kinetex

Part No

00D-4759-AN

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: 2mM Ammonium acetate (pH 6.9)

B: Methanol/Acetonitrile (50-50)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

55

45

2

9

30

70

3

9.5

30

70

4

9.51

55

45

5

12

55

45

Flow Rate

400.00 mL/min

Temperature

50°C

Detection

Mass Spectrometer (MS) @ (50°C)

Detection Info