12 Cannabninoids in AcCN using Kinetex 2.6um Polar C18 150x4.6mm

LC Conditions

Column

Kinetex 2.6 µm Polar C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4759-E0

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% phosphoric acid

B: Acetonitrile with 0.1% phosphoric acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

25

75

2

6

0

100

Flow Rate

1.80 mL/min

Temperature

50°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info