Water Soluble Vitamins on Kinetex 2.6u Polar 150x4.6mm

LC Conditions

Column

Kinetex 2.6 µm Polar C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4759-E0

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: 20mM Potassium Phosphate pH 3.5

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

10

70

30

3

11

70

30

4

11.5

100

0

5

14

100

0

Flow Rate

1.20 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info