Vitamin B2 and B3 Extracted from Human Serum

LC Conditions

Column

Luna Omega 3 µm Polar C18 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00B-4760-E0

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: 10 mM Ammonium formate/w 0.1%FA

B: 0.1% FA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.01

98

2

2

0.5

98

2

3

3

10

90

4

3.5

10

90

5

3.51

98

2

6

5

98

2

Flow Rate

750.00 mL/min

Temperature

21°C

Detection

Tandem Mass Spec (MS-MS) @ (650°C)

Detection Info