Pantothenic acid (Vit B5) in human serum

LC Conditions

Column

Luna Omega 3 µm Polar C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Luna Omega

Part No

00B-4760-AN

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: 10 mM Ammonium formate/w 0.1%FA

B: 0.1% FA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.01

100

0

2

0.6

100

0

3

3

10

90

4

3.5

10

90

5

3.6

100

0

6

5

100

0

Flow Rate

500.00 mL/min

Temperature

21°C

Detection

Tandem Mass Spec (MS-MS) @ (650°C)

Detection Info