Plasma catecholamines using Luna Omega 3um Polar C18 50 x 2.1mm

LC Conditions

Column

Luna Omega 3 µm Polar C18 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00B-4760-E0

Phase Name

Polar C18

Elution Type

Gradient

Mobile Phase

A: 10mM ammonium acetate

B: Acetonitrile with 0.1% formic acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

0

2

3

0

100

Flow Rate

0.40 mL/min

Temperature

22°C

Detection

Electrospray Mass Spec (ESMS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-CW Polymeric Weak Cation, microelution 96-well plate, 2mg/well, EA

Part No

8M-S035-4GA

Pretreatment Note

Pool eluents and dilute with 100 µL of 0.1% Formic acid in water

Condition 1

0.2 mL Methanol

Condition 2

0.2 mL 50 mM Ammonium acetate, pH 7

Load

Load 1.00 mL Sample diluted in Pretreated Sample

Wash 1

0.2 mL 50 mM Ammonium acetate, pH 7

Wash 2

0.2 mL Acetonitrile / Isopropyl alcohol (1:1)

Dry

3 minutes under high vacuum

Elution 1

0.025 mL Water / Acetonitrile / Formic acid (85:10:5)

Elution 2

0.025 mL Water / Acetonitrile / Formic acid (85:10:5)

Evaporation

Evaporate to dryness