Overlay PPT/SLE/MeOHVitD PCI

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% formic acid in water

B: 0.1% formic acid in acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

40

60

2

1

0

100

3

7

0

100

4

7.1

40

60

5

10

40

60

Flow Rate

300.00 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info