Batch To Batch Reproducibility on bioZen Intact C4

Batch To Batch Reproducibility on bioZen Intact C4
LC Conditions
Column

bioZen 3.6 µm Intact C4, LC Column 150 x 4.6 mm, Ea

Brand

Biozen LC

Part No

00F-4767-E0

Phase Name

Intact C4

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: 0.1% TFA in ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

0

70

30

3

0

50

50

4

0

90

10

Flow Rate

1.50 mL/min

Temperature

40°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Order Items Used in This Application

  • 1. Ligh Chain
  • 2. Heavy Chain
  • 3. Intact mAb
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