Intact Mass- NIST mAb C4

LC Conditions

Column

bioZen 3.6 µm Intact C4, LC Column 150 x 2.1 mm, Ea

Brand

Biozen LC

Part No

00F-4767-AN

Phase Name

Intact C4

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1 % Formic Acid in ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

3

85

15

3

9

10

90

4

11.4

10

90

5

11.5

15

85

6

15

15

85

Flow Rate

0.50 mL/min

Temperature

80°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info