IntantPC Labeled N-Linked Glycans on Trastuzumab, Innovator on bioZen Glycan

LC Conditions

Column

bioZen 2.6 µm Glycan, LC Column 150 x 2.1 mm, Ea

Brand

Biozen LC

Part No

00F-4773-AN

Phase Name

Glycan

Elution Type

Gradient

Mobile Phase

A: 100 mM Ammonium Formate, pH 4.5

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

22

78

2

10

25.5

74.5

3

24

28

72

4

38.5

44.1

55.9

5

38.6

60

40

6

40.6

60

40

7

40.7

22

78

8

48

22

78

Flow Rate

0.50 mL/min

Temperature

50°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

LC/FLD (Fluorescence Detector) @ 285 nm (nanometers)

Detection Info

Sample Notes

Utilize bioZen N-Glycan Clean-Up microelution SPE 96well plate for sample preparation (Part No.: 8M-S009-NGA).