Infliximab Peptide Mapping with Tryptic Digest on bioZen 2.6 µm Peptide XB-C18

LC Conditions

Column

bioZen 2.6 µm Peptide XB-C18, LC Column 150 x 2.1 mm, Ea

Brand

Biozen LC

Part No

00F-4768-AN

Phase Name

PEPTIDE XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid in water

B: 0.1% Formic acid in acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

99

1

2

0.5

99

1

3

50

50

50

4

55

50

50

5

56

5

95

Flow Rate

0.30 mL/min

Temperature

40°C

System

Agilent Technologies 1290 Infinity SCIEX X500B Q-TOF

Detection

LC/MS

Detection Info

Sample Notes

1% acetonitrile, 0.1% formic acid in water