Charge Variants of Cetuximab using Salt Gradient on bioZen 6 µm WCX

LC Conditions

Column

bioZen 6µm WCX, LC Column 250 x 4.6 mm, Ea

Brand

Biozen LC

Part No

00G-4777-E0

Phase Name

WCX

Elution Type

Gradient

Mobile Phase

A: 20 mM MES, pH 5.6

B: 20 mM MES, 300 mM NaCl, pH 5.6

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

1

85

15

3

31

55

45

4

31.1

0

100

5

34

0

100

6

35

85

15

Flow Rate

1.00 mL/min

Temperature

25°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC H-Class

Detection

LC/UV (DAD, PDA) @ 280 nm (nanometers)

Detection Info

Sample Notes

*Sample graciously gifted by Catherine Bladen, Absolute Antibody