Nimesulide in formulation gel using Kinetex 2.6µm Polar C18 150x.4.6mm

LC Conditions

Column

Kinetex 2.6 µm Polar C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4759-E0

Phase Name

Polar C18

Guard Cartridge:

AJ0-9530

Guard Holder:

AJ0-9000

Elution Type

Gradient

Mobile Phase

A: 30mM Potassium Phosphate pH 3.0

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

50

50

2

8

20

80

3

9

20

80

4

10

50

50

5

12

50

50

Flow Rate

1.00 mL/min

Temperature

40°C

System

Waters ACQUITY UPLC H-Class Waters ACQUITY UPLC I-Class

Detection

LC/UV (DAD, PDA) @ 254 nm (nanometers) (30°C)

Detection Info

Sample Notes

Gel formulation extraction