Dicamba and Acidic Herbicides LC-MS/MS in Agricultural Field Samples
LC Conditions
Column
Kinetex 2.6 µm F5 100 Å, LC Column 100 x 3.0 mm, Ea
Brand
Kinetex
Part No
00D-4723-Y0
Phase Name
F5
Elution Type
Gradient
Mobile Phase
A: water
B: 0.2% formic acid in methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
98
2
2
1
60
40
3
4
48
52
4
12
15
85
5
13.5
10
90
6
15.5
10
90
7
15.6
98
2
Flow Rate
0.50 mL/min
Temperature
30°C
System
Sciex SCIEX 6500+ QTRAP
Detection
LC/MS/MS
Detection Info
Sample Notes
5 g of soil sample or soybean foliage were collected from impacted and non-impacted agricultural field sites. Internal standard was added to the sample pre-extraction. Sample was homogenized and extracted with formic acid fortified acetonitrile. Sample was shaken for 15 minutes then centrifuged at 4000 rpm. The supernatant was diluted with aqueous mobile phase into 2 mL amber autosampler vials for LC-MS/MS analysis.
Order Items Used in This Application
- 1. 13C6-DCSA
- 2. 2,4,5-T_1
- 3. 2,4,5-T_2
- 4. 2,4,5-T_3
- 5. 2,4,5-TP_1
- 6. 2,4,5-TP_2
- 7. 2,4-D_1
- 8. 2,4-D_2
- 9. 2,4-D_3
- 10. 2,4-D_4
- 11. 2,4-DB_1
- 12. 2,4-DB_2
- 13. 2,4-DB_3
- 14. 2,4-DB_4
- 15. 5-OH-Dicamba_1
- 16. 5-OH-Dicamba_2
- 17. 5-OH-Dicamba_3
- 18. 5-OH-Dicamba_4
- 19. Acifluorfen_1
- 20. Acifluorfen_2
- 21. Acifluorfen_3
- 22. Acifluorfen_4
- 23. Bentazon_1
- 24. Bentazon_3
- 25. Bentazon_4
- 26. d3-Dicamba_1
- 27. d3-Dicamba_2
- 28. DCGA_1
- 29. DCGA_2
- 30. DCGA_3
- 31. DCGA_4
- 32. DCSA_1
- 33. DCSA_2
- 34. DCSA_3
- 35. DCSA_4
- 36. Dicamba_1
- 37. Dicamba_2
- 38. Dicamba_3
- 39. Dichlorprop_1
- 40. Dichlorprop_2
- 41. Dichlorprop_3
- 42. Dichlorprop_4
- 43. MCPA_1
- 44. MCPA_2
- 45. MCPA_3
- 46. MCPB_1
- 47. MCPB_2
- 48. MCPP_1
- 49. MCPP_2
- 50. MCPP_3
Similar Applications
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