Benzalkonium Chloride UPS method using Kinetex 2.6u Biphenyl 100x2.1mm

LC Conditions

Column

Kinetex 2.6 µm Biphenyl 100 Å, LC Column 100 x 2.1 mm, Ea

Brand

Kinetex

Part No

00D-4622-AN

Phase Name

Biphenyl

Guard Cartridge:

AJ0-4304

Guard Holder:

KJ0-4282

Elution Type

Isocratic

Mobile Phase

A: Water with 100 mM Ammonium Acetate and 0.2 % Phosphoric Acid

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

5

35

65

Flow Rate

0.30 mL/min

Temperature

30°C

System

Waters ACQUITY UPLC I-Class Waters ACQUITY UPLC I-Class

Detection

LC/UV (DAD, PDA) @ 214 nm (nanometers) (20°C)

Detection Info

Syringe Filter

AF0-2207-12