16 Cannabinoids on Kinetex C18

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% formic acid in water

B: 0.1% formic acid in 75:25 Methanol:Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

20

80

2

8

20

80

3

10

0

100

4

12

0

100

Flow Rate

1.50 mL/min

Temperature

30°C

System

1200 Series high performance autosampler, 0.1 100 µL Agilent Technologies 1200 Series

Detection

LC/UV (DAD, PDA) @ 228

Detection Info