22 Antibiotics by LCMS on Kinetex 2.6 µm PS C18 50x2.1mm

LC Conditions

Column

Kinetex 2.6 µm PS C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4780-AN

Phase Name

PS C18

Guard Cartridge:

AJ0-8951

Guard Holder:

AJ0-9000

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

0.5

97

3

3

5

0

100

4

5.1

97

3

5

8

97

3

Flow Rate

500.00 mL/min

Temperature

30°C

System

Agilent Technologies 1200 Series SCIEX API 4000

Detection

LC/MS @ (30°C)

Detection Info