Aloe Emodin, Eugenol Methyl Ether, and Aloins (A and B) from Aloe Standards Screen on Kinetex 2.6u PS C18 50x4.6mm

LC Conditions

Column

Kinetex 2.6 µm PS C18 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Kinetex

Part No

00B-4780-E0

Phase Name

PS C18

Guard Cartridge:

AJ0-8949

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

2

0.5

75

25

3

5

20

80

4

7

20

80

5

7.1

75

25

6

9

75

25

Flow Rate

1.20 mL/min

Temperature

30°C

System

Agilent Technologies 1100 Series Agilent Technologies 1100 Series

Detection

LC/UV (DAD, PDA) @ 220 nm (nanometers) (30°C)

Detection Info