Bile Acids by LCMS, Kinetex 2.6u PS C18 100x2.1mm (Murideoxycholic acid and Hyodeoxycholic acid)

LC Conditions

Column

Kinetex 2.6 µm PS C18 100 Å, LC Column 100 x 2.1 mm, Ea

Brand

Kinetex

Part No

00D-4780-AN

Phase Name

PS C18

Guard Cartridge:

AJ0-8951

Elution Type

Gradient

Mobile Phase

A: 10mM Ammonium Acetate in Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

6

60

40

3

6.1

80

20

4

9

80

20

Flow Rate

500.00 mL/min

Temperature

30°C

System

Agilent Technologies 1200 Series SCIEX API 4000

Detection

LC/MS @ (30°C)

Detection Info