Imipramine and Amitriptyline Loading on Kinetex 2.6µm PS C18 50x4.6mm using Waters ACQUITY I Class UPLC
LC Conditions
Column
Kinetex 2.6 µm PS C18 100 Å, LC Column 50 x 4.6 mm, Ea
Brand
Kinetex
Part No
00B-4780-E0
Phase Name
PS C18
Guard Cartridge:
AJ0-8949
Guard Holder:
AJ0-9000
Elution Type
Gradient
Mobile Phase
A: Methanol
B: 0.1% Formic Acid in ACN
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
75
25
2
2
65
35
3
3
5
95
4
3.1
75
25
5
5
75
25
Flow Rate
1.85 mL/min
Temperature
30°C
System
Waters ACQUITY UPLC I-Class Waters ACQUITY UPLC I-Class
Detection
LC/UV (DAD, PDA) @ 254 (30°C)
Detection Info
Sample Notes
Imipramine (0.5 mg/mL), with various concentrations of Amitriptyline (0.5, 2.5, 5, and 10%) prepared in water
Order Items Used in This Application
- 1. Imipramine
- 2. Amitriptyline
-OEChem-06211003152D<br><br> 45 47 0 0 0 0 0 0 0999 V2000<br> 4.8830 0.9047 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1509 -2.0953 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8830 -0.0953 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.9820 1.3386 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7840 1.3385 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3830 3.0953 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3830 3.0953 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7595 2.3135 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0065 2.3135 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0170 -0.5953 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0169 -1.5953 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.2295 0.6184 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5365 0.6184 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.7690 2.6358 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9970 2.6358 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2318 0.9177 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.5342 0.9177 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 1.9333 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7660 1.9332 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1509 -3.0953 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2849 -1.5953 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0950 -0.6779 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4936 0.0123 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5210 3.6998 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8244 3.3643 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.9416 3.3643 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2450 3.6998 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8049 -0.0127 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4064 -0.7030 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2290 -2.1779 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6275 -1.4877 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3744 0.0156 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3916 0.0155 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.6380 3.2418 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1280 3.2418 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.7789 0.4943 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9871 0.4942 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4083 2.1183 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.3578 2.1182 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5309 -3.0953 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1509 -3.7153 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7709 -3.0953 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5949 -1.0584 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.7480 -1.2853 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.9749 -2.1322 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 3 1 0 0 0 0<br> 1 4 1 0 0 0 0<br> 1 5 1 0 0 0 0<br> 2 11 1 0 0 0 0<br> 2 20 1 0 0 0 0<br> 2 21 1 0 0 0 0<br> 3 10 1 0 0 0 0<br> 3 22 1 0 0 0 0<br> 3 23 1 0 0 0 0<br> 4 8 1 0 0 0 0<br> 4 12 2 0 0 0 0<br> 5 9 1 0 0 0 0<br> 5 13 2 0 0 0 0<br> 6 7 1 0 0 0 0<br> 6 8 1 0 0 0 0<br> 6 24 1 0 0 0 0<br> 6 25 1 0 0 0 0<br> 7 9 1 0 0 0 0<br> 7 26 1 0 0 0 0<br> 7 27 1 0 0 0 0<br> 8 14 2 0 0 0 0<br> 9 15 2 0 0 0 0<br> 10 11 1 0 0 0 0<br> 10 28 1 0 0 0 0<br> 10 29 1 0 0 0 0<br> 11 30 1 0 0 0 0<br> 11 31 1 0 0 0 0<br> 12 16 1 0 0 0 0<br> 12 32 1 0 0 0 0<br> 13 17 1 0 0 0 0<br> 13 33 1 0 0 0 0<br> 14 18 1 0 0 0 0<br> 14 34 1 0 0 0 0<br> 15 19 1 0 0 0 0<br> 15 35 1 0 0 0 0<br> 16 18 2 0 0 0 0<br> 16 36 1 0 0 0 0<br> 17 19 2 0 0 0 0<br> 17 37 1 0 0 0 0<br> 18 38 1 0 0 0 0<br> 19 39 1 0 0 0 0<br> 20 40 1 0 0 0 0<br> 20 41 1 0 0 0 0<br> 20 42 1 0 0 0 0<br> 21 43 1 0 0 0 0<br> 21 44 1 0 0 0 0<br> 21 45 1 0 0 0 0<br>M END
Compound Name
Imipramine
CID
3696
Molecular Formula
C19H24N2
Molecular Weight
280
No. Hydrogen Bond Acceptors
2
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