Berberine extracted from Goldenseal pill on Kinetex PS-C18

LC Conditions

Column

Kinetex 2.6 µm PS C18 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Kinetex

Part No

00B-4780-E0

Phase Name

PS C18

Guard Cartridge:

AJ0-8949

Elution Type

Gradient

Mobile Phase

A: 20mM Potassium dihydrogen phosphate pH 4.6

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

7

35

65

3

8

35

65

4

8.5

90

10

5

10

90

10

Flow Rate

1.50 mL/min

Temperature

23°C

System

Agilent Technologies 1100 Series Agilent Technologies 1100 Series

Detection

LC/UV (DAD, PDA) @ 235 nm (nanometers)

Detection Info

Sample Notes

pill crushed and dissolved in 10mls 50:50 methanol:20 mM phosphate buffer. Sample vortexed and centrifuged. Supernatant transferred to HPLC vial