Water-Soluble Vitamins

LC Conditions

Column

Luna 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4251-E0

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: Methanol

B: 0.005 M Hexanesulfonic Acid (0.1% TFA and 0.01% TEA) with 5 % Methanol premixed

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

0

100

2

5

0

100

3

16

23

77

4

26

26

74

5

28

50

50

6

30

0

100

7

45

0

100

Flow Rate

0.55 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282