Metformin Dissolution Test 3 with Prodigy ODS-3 (RS)
LC Conditions
Column
Prodigy 5 µm ODS-3 100 Å, LC Column 250 x 4.6 mm, Ea
Brand
Prodigy
Part No
00G-4097-E0
Phase Name
ODS-3
Elution Type
Isocratic
Mobile Phase
A: 1.38 g of monobasic sodium phosphate in about 1800 mL of water
3.484 g of 1-pentanesulfonic acid sodium salt added, then adjust pH to 3.00 ± 0.05 with diluted phosphoric acid and bring to 2000 mL in a volumetric flask
B: Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
15
95
5
Flow Rate
1.00 mL/min
Temperature
25°C
System
Agilent Technologies Agilent Technologies 1100 Series
Detection
LC/UV (DAD, PDA) @ 230 nm (nanometers)
Detection Info
Syringe Filter
AF0-2107-12
Sample Notes
Acetonitrile and Buffer (1:19) equivalent to 95% A (buffer) / 5% B (Acetonitrile)
Order Items Used in This Application
- 1. Metformin
-OEChem-06211003152D<br><br> 22 20 0 0 0 0 0 0 0999 V2000<br> 2.2020 0.0000 0.0000 Cl 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0010 4.3100 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2690 4.3100 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1350 2.8100 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 0.5369 4.3100 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4030 2.8100 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8671 3.8100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0010 5.3100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1350 3.8100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4030 3.8100 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5571 3.2731 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4040 3.5000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1771 4.3469 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6210 5.3100 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0010 5.9300 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3810 5.3100 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.6720 2.5000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 0.0000 4.0000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 0.5369 4.9300 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 0.8660 2.5000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.9399 2.5000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.2020 0.0000 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 22 1 0 0 0 0<br> 2 7 1 0 0 0 0<br> 2 8 1 0 0 0 0<br> 2 9 1 0 0 0 0<br> 3 9 1 0 0 0 0<br> 3 10 2 0 0 0 0<br> 4 9 2 0 0 0 0<br> 4 17 1 0 0 0 0<br> 5 10 1 0 0 0 0<br> 5 18 1 0 0 0 0<br> 5 19 1 0 0 0 0<br> 6 10 1 0 0 0 0<br> 6 20 1 0 0 0 0<br> 6 21 1 0 0 0 0<br> 7 11 1 0 0 0 0<br> 7 12 1 0 0 0 0<br> 7 13 1 0 0 0 0<br> 8 14 1 0 0 0 0<br> 8 15 1 0 0 0 0<br> 8 16 1 0 0 0 0<br>M END
Compound Name
Metformin
CID
14219
Molecular Formula
C4H12ClN5
Molecular Weight
165
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
4
Similar Applications
Reversed Phase
Metformin Dissolution Test 3 with Prodigy ODS-3 (RS)
Prodigy 5 µm ODS-3 100 Å, LC Column 250 x 4.6 mm, Ea
25540