Proteins

LC Conditions

Column

Luna 5 µm C8(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4249-E0

Phase Name

C8(2)

Elution Type

Gradient

Mobile Phase

A: 0.085% Phosphoric acid, 0.3% TEA, pH 7.0 with NaOH

B: Solution A/Acetonitrile (40/60)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

55

45

2

30

32

68

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290 - Holder Part No.: KJ0-4282