Fat-Soluble Vitamins

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4252-E0

Phase Name

C18(2)

Elution Type

Gradient

Mobile Phase

A: Acetonitrile

B: Water

C: Methanol/THF (90:10)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

15

7

2

25

15

7

3

25.1

25

0

4

45

25

0

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282