Intact Reversed Phase Analysis of NIST mAb-Gradient Optimization (Detailed Overlay)

LC Conditions

Column

bioZen 2.6 µm WidePore C4, LC Column 100 x 2.1 mm, Ea

Brand

Biozen LC

Part No

00D-4786-AN

Phase Name

WIDEPORE C4

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: 0.1% TFA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

6

45

55

3

6.1

10

90

4

6.3

80

20

5

6.5

10

90

6

6.7

80

20

7

6.9

10

90

8

7.1

80

20

9

8

80

20

Flow Rate

0.50 mL/min

Temperature

90°C

System

Waters Waters ACQUITY UPLC I-Class

Detection

LC/UV (DAD, PDA) @ 280 nm (nanometers)

Detection Info