Reduced NIST mAb on bioZen 2.6 µm WidePore C4

LC Conditions

Column

bioZen 2.6 µm WidePore C4, LC Column 100 x 2.1 mm, Ea

Brand

Biozen LC

Part No

00D-4786-AN

Phase Name

WIDEPORE C4

Elution Type

Gradient

Mobile Phase

A: 0.1 % TFA in Water

B: 0.1 % TFA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

6

45

55

3

6.1

10

90

4

6.3

10

90

5

6.7

80

20

Flow Rate

0.50 mL/min

Temperature

90°C

System

Waters ACQUITY UPLC I-Class Waters ACQUITY UPLC I-Class

Detection

LC/UV (DAD, PDA)

Detection Info