Chromatographic Performance for Intact Mass Applications, Comparison of 4 mAbs

LC Conditions

Column

bioZen 2.6 µm WidePore C4, LC Column 100 x 2.1 mm, Ea

Brand

Biozen LC

Part No

00D-4786-AN

Phase Name

WIDEPORE C4

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

4

10

90

3

4.1

95

5

4

4.3

10

90

5

4.5

95

5

6

4.7

90

10

Flow Rate

0.30 mL/min

Temperature

60°C

System

Waters ACQUITY UPLC I-Class Waters ACQUITY UPLC I-Class

Detection

LC/UV (DAD, PDA)

Detection Info