Chloroquine Phosphate Tablet - USP Assay on Luna 5 µm C18(2) 150 x 4.6 mm

LC Conditions

Column

Luna 5 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna

Part No

00F-4252-E0

Phase Name

C18(2)

Elution Type

Isocratic

Mobile Phase

A: Methanol and Buffer (22:78) Buffer: 6.8 g/L of monobasic potassium phosphate in water. Add 1.0 mL of perchloric acid to each 1 L of solution, adjust with phosphoric acid to a pH of 2.5

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

Flow Rate

1.20 mL/min

Temperature

26°C

System

Agilent Technologies 1200 Series high performance autosampler, 0.1 100 µL

Detection

LC/UV (DAD, PDA) @ 224 nm (nanometers)

Detection Info

Sample Notes

system suitability solution (0.15 mg/mL USP Chloroquine phosphate RS and 0.15 mg/mL USP Amodiaquine hydrochloride RS in water)