Fast Screening Method for 86 Forensic Analytes in Human Urine by LC-MS/MS
LC Conditions
Column
Luna 5 µm C18(2) 100 Å, LC Column 50 x 2 mm, Ea
Brand
Luna
Part No
00B-4252-B0
Phase Name
C18(2)
Elution Type
Gradient
Mobile Phase
A: 5 mM Ammonium formate in water
B: 0.1 % Formic acid in methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
95
5
2
1.8
5
95
Flow Rate
0.20 mL/min
Temperature
22°C
System
Sciex Applied Biosystems Q TRAP, Mass Analyzer: QQQ-LT, Interface(s): ES/APCI, PI
Detection
LC/MS/MS
Detection Info
Sample Notes
Hydrolyze 100 µL urine by adding 25 µL IMCS RapidHydrolysis Buffer, 20 µL IMCSzyme, and 10 µL IS. All to hydrolyze for 30 to 60 min at 55 °C. After hydrolysis, add 0.8 mL of diluent to the mixture and centrifuge at 21,000 g for 15 min. Transfer supernatant to a glass vial with insert for analysis by LC-MS/MS.
Order Items Used in This Application
- 1. 6-MAM
- 2. 7-Aminoclonazepam
- 3. 7-Hydroxymitragynine
- 4. Acetyl Fentanyl
- 5. Alpha-Hydroxyalprazolam
- 6. Alpha-Hydroxymidazolam
- 7. Alpha-Hydroxytriazolam
- 8. Alpha-PPP
- 9. Alpha-PVP
- 10. Alprazolam
- 11. AM-2201 4-OH pentyl
- 12. Amitriptyline
- 13. Amphetamine
- 14. Benzoylecgonine
- 15. Buphedrone
- 16. Buprenorphine
- 17. Carisoprodol
- 18. Clomipramine
- 19. Codeine
- 20. Cotinine
- 21. Cyclobenzaprine
- 22. Desalkylflurazepam
- 23. Desipramine
- 24. Desmethyldoxepin
- 25. Dextromethorphan
- 26. Diazepam
- 27. Dihydrocodeine
- 28. Doxepin
- 29. EDDP
- 30. Fentanyl
- 31. Gabapentin
- 32. Hydrocodone
- 33. Hydromorphone
- 34. Imipramine
- 35. JWH-018 4-OH pentyl
- 36. JWH-018 pentanoic acid
- 37. JWH-019 6-OH hexyl
- 38. JWH-073 3-OH butyl
- 39. JWH-073 butanoic acid
- 40. JWH-081 5-OH pentyl
- 41. JWH-122 5-OH pentyl
- 42. JWH-210 5-OH pentyl
- 43. JWH-250 4-OH pentyl
- 44. Lorazepam
- 45. MDA
- 46. MDEA
- 47. MDMA
- 48. MDPV
- 49. Meperidine
- 50. Mephedrone
- 51. Meprobamate
- 52. Methadone
- 53. Methamphetamine
- 54. Methedrone
- 55. Methylone
- 56. Methylphenidate
- 57. Midazolam
- 58. Mitragynine
- 59. Morphine
- 60. Naloxone
- 61. Naltrexone
- 62. N-desmethyltapentadol
- 63. Norbuprenorphine
- 64. Norcodeine
- 65. Nordiazepam
- 66. Norfentanyl
- 67. Norhydrocodone
- 68. Normeperidine
- 69. Noroxycodone
- 70. Norpropoxyphene
- 71. Nortriptyline
- 72. O-Desmethyltramadol
- 73. Oxazepam
- 74. Oxycodone
- 75. Oxymorphone
- 76. PCP
- 77. Pregabalin
- 78. Propoxyphene
- 79. Protriptyline
- 80. RCS4-4-OH-pentyl
- 81. Ritalinic Acid
- 82. Sufentanil
- 83. Tapentadol
- 84. Temazepam
- 85. Tramadol
- 86. Zolpidem
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