Identification and Quantification of Designer Drugs in Urine by LC-MS/MS

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 3 mm, Ea

Brand

Kinetex

Part No

00F-4462-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Ammonium formate in water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

6

5

95

3

6.2

5

95

4

8

95

5

Flow Rate

0.50 mL/min

Temperature

50°C

System

Sciex Applied Biosystems Q TRAP,  Mass Analyzer:  QQQ-LT, Interface(s): ES/APCI, PI

Detection

LC/MS/MS

Detection Info

Sample Notes

To 20 µL urine, add 50 µL internal standard (D5-PCP in acetonitrile). Vortex then centrifuge for 10 min at 15,000 rpm. Dilute supernatant with 930 µL Mobile Phase A. Mix and transfer to an autosampler vial for injection.