Analysis of Chloroquine and Its Metabolite by LC-MS/MS using Kinetex® Core-Shell F5 LC Columns

LC Conditions

Column

Kinetex 2.6 µm F5 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4723-AN

Phase Name

F5

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

2

93

7

3

3

85

15

4

3.2

60

40

5

4.2

5

95

6

4.3

98

2

7

6

98

2

Flow Rate

0.50 mL/min

Temperature

20°C

System

Agilent Technologies 1200 Series SCIEX API 4000 QTRAP

Detection

LC/MS/MS

Detection Info