USP Chloroquine Phosphate Assay on Kinetex 5 µm C18 150 x 4.6 mm - Standard Solution

LC Conditions

Column

Kinetex 5 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4601-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: A = 0.4% triethylamine in methanol and Buffer (70:30) (Buffer: 1.4 g/L of anhydrous dibasic sodium phosphate in water. Adjust with 10% phosphoric acid to a pH of 3.0)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

100

2

20

100

Flow Rate

1.00 mL/min

Temperature

26°C

System

Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity

Detection

LC/UV (DAD, PDA) @ 260 nm (nanometers) (26°C)

Detection Info

Sample Notes

Sample was at 0.3 mg/mL