Sildenafil Citrate USP Diluted Standard Solution 0.0014 mg/mL on Kinetex 5 µm C18 150x4.6mm LC/UV

LC Conditions

Column

Kinetex 5 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4601-E0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: Buffer (dilute 7 mL of triethylamine with water to 1 L. Stir and adjust with phosphoric acid to a pH of 3.0+/- 0.1), Methanol and Acetonitrile (58:25:17)

Gradient Profile

Step No.

Time(min)

%A

%B

1

20

100

Flow Rate

1.40 mL/min

Temperature

30°C

System

Agilent Technologies 1100 Series Agilent Technologies 1100 Series

Detection

LC/UV (DAD, PDA) @ 290 nm (nanometers) (30°C)

Detection Info

Sample Notes

0.0014 mg/mL