Dexamethasone USP System Suitability Solution on Kinetex 1.7 µm C18 100 x 2.1 mm on Agilent 1290 UHPLC
LC Conditions
Column
Kinetex 1.7 µm C18 100 Å, LC Column 100 x 2.1 mm, Ea
Brand
Kinetex
Part No
00D-4475-AN
Phase Name
C18
Elution Type
Gradient
Mobile Phase
A: 3.4 g/L of monobasic potassium phosphate in water. Adjust with phosphoric acid to a pH of 3.0
B: Acetonitrile
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
76
24
2
10
76
24
3
15
45
55
4
16
10
90
5
16.1
76
24
6
20
76
24
Flow Rate
0.40 mL/min
Temperature
35°C
System
Agilent Technologies Agilent Technologies 1290 Infinity
Detection
LC/UV (DAD, PDA) @ 240 nm (nanometers)
Detection Info
Order Items Used in This Application
- 1. Betamethasone
- 2. Dexamethasone
-OEChem-06211003152D<br><br> 57 60 0 1 0 0 0 0 0999 V2000<br> 5.0182 -0.2293 0.0000 F 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0319 1.2610 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7950 0.5046 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.0622 0.4874 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.8566 2.4768 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -3.0477 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3931 0.0046 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 7.3931 -0.9954 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 6.5271 -1.4954 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 5.6610 -0.9954 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 8.3393 0.3094 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 8.3393 -1.3001 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5271 0.5046 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9229 -0.4954 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 5.6610 0.0046 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 4.7510 -1.5022 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 6.5431 -2.5369 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3931 1.0046 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6451 -3.0646 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7430 -2.5438 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.1493 0.8957 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9229 -0.4954 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.4163 -0.5599 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8242 -0.9381 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8076 -3.0935 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.0465 1.8904 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8763 -1.4661 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8680 -2.5510 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4830 -1.8406 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2664 -1.9147 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0883 -1.8670 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8767 -1.6093 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9256 0.9796 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1285 0.9796 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.2046 -1.0477 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6610 0.6246 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.7612 -3.1173 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1523 -2.4214 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0131 1.0046 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3931 1.6246 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.7731 1.0046 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2478 -3.5406 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0460 -3.5375 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9229 -1.1154 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5429 -0.4954 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9229 0.1246 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.0005 -0.3524 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2087 0.0243 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8320 -0.7675 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.4476 1.7210 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8361 -0.3183 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7950 1.1246 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8100 -3.7135 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.7757 2.4482 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.4502 1.7206 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3430 -1.1499 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.7928 3.0935 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10 1 1 6 0 0 0<br> 11 2 1 6 0 0 0<br> 2 50 1 0 0 0 0<br> 15 3 1 1 0 0 0<br> 3 52 1 0 0 0 0<br> 4 21 2 0 0 0 0<br> 5 26 1 0 0 0 0<br> 5 57 1 0 0 0 0<br> 6 28 2 0 0 0 0<br> 7 8 1 0 0 0 0<br> 7 11 1 0 0 0 0<br> 7 13 1 0 0 0 0<br> 7 18 1 1 0 0 0<br> 8 9 1 0 0 0 0<br> 8 12 1 0 0 0 0<br> 8 29 1 6 0 0 0<br> 9 10 1 0 0 0 0<br> 9 17 1 0 0 0 0<br> 9 30 1 1 0 0 0<br> 10 15 1 0 0 0 0<br> 10 16 1 0 0 0 0<br> 11 14 1 0 0 0 0<br> 11 21 1 0 0 0 0<br> 12 14 1 0 0 0 0<br> 12 31 1 0 0 0 0<br> 12 32 1 0 0 0 0<br> 13 15 1 0 0 0 0<br> 13 33 1 0 0 0 0<br> 13 34 1 0 0 0 0<br> 14 22 1 1 0 0 0<br> 14 35 1 0 0 0 0<br> 15 36 1 0 0 0 0<br> 16 20 1 0 0 0 0<br> 16 23 1 1 0 0 0<br> 16 24 1 0 0 0 0<br> 17 19 1 0 0 0 0<br> 17 37 1 0 0 0 0<br> 17 38 1 0 0 0 0<br> 18 39 1 0 0 0 0<br> 18 40 1 0 0 0 0<br> 18 41 1 0 0 0 0<br> 19 20 1 0 0 0 0<br> 19 42 1 0 0 0 0<br> 19 43 1 0 0 0 0<br> 20 25 2 0 0 0 0<br> 21 26 1 0 0 0 0<br> 22 44 1 0 0 0 0<br> 22 45 1 0 0 0 0<br> 22 46 1 0 0 0 0<br> 23 47 1 0 0 0 0<br> 23 48 1 0 0 0 0<br> 23 49 1 0 0 0 0<br> 24 27 2 0 0 0 0<br> 24 51 1 0 0 0 0<br> 25 28 1 0 0 0 0<br> 25 53 1 0 0 0 0<br> 26 54 1 0 0 0 0<br> 26 55 1 0 0 0 0<br> 27 28 1 0 0 0 0<br> 27 56 1 0 0 0 0<br>M END
Compound Name
Betamethasone
CID
9782
Molecular Formula
C22H29FO5
Molecular Weight
392
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
3
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