USP Butamben Impurity Standard Solution 0.2 µg/mL on Kinetex 5 µm XB-C18 100 x 2.1 mm on Agilent 1260 UHPLC

LC Conditions

Column

Kinetex 5 µm XB-C18 100 Å, LC Column 100 x 2.1 mm, Ea

Brand

Kinetex

Part No

00D-4605-AN

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

1

90

10

3

6

50

50

4

8.5

50

50

5

8.6

90

10

6

10

90

10

Flow Rate

0.40 mL/min

Temperature

30°C

System

Agilent Technologies Agilent Technologies 1200 Series Binary LC System

Detection

LC/UV (DAD, PDA) @ 285 nm (nanometers)

Detection Info