Hair Colorants (Dyes) on Bondclone 10u C18

LC Conditions

Column

Bondclone 10 µm C18 148 Å, LC Column 300 x 3.9 mm, Ea

Brand

Bondclone

Part No

00H-2117-C0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: 0.005 M Diaminooctane-0.020 M Heptanesulfonate pH 4.5

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

HPLC from a commerical hair colorant preparation (paste) with the fotoreactor off (A) and on (B). SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282