USP Doxepin Assay - Standard Solution on Kinetex® 2.6 µm C8 75 x 4.6 mm

LC Conditions

Column

Kinetex 2.6 µm C8 100 Å, LC Column 75 x 4.6 mm, Ea

Brand

Kinetex

Part No

00C-4497-E0

Phase Name

C8

Elution Type

Isocratic

Mobile Phase

A: 27.6 g/L monobasic sodium phosphate, pH 2.5

B: methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

70

30

Flow Rate

1.32 mL/min

Temperature

50°C

System

Agilent Technologies 1290 Infinity Agilent Technologies 1290 Infinity

Detection

LC/UV (DAD, PDA) @ 254 nm (nanometers)

Detection Info

Sample Notes

Standard solution/sample solution: 0.1 mg/mL of USP Doxepin Hydrochloride RS in Mobile phase.