USP Alosetron Tablet Assay Standard/Sample solution (0.0115 mg/mL) on Gemini 3 µm C18 75 x 4.6 mm using Agilent 1260 Binary UHPLC

LC Conditions

Column

Gemini 3 µm C18 110 Å, LC Column 75 x 4.6 mm, Ea

Brand

Gemini

Part No

00C-4439-E0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: 0.02 M monobasic sodium phosphate. Adjust with phosphoric acid to a pH of 3.0

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

10

80

20

Flow Rate

1.00 mL/min

Temperature

25°C

System

Agilent Technologies Agilent Technologies 1260 Infinity

Detection

LC/UV (DAD, PDA) @ 220 nm (nanometers)

Detection Info