Steroid mix on Luna Omega 3 µm C18 150x4.6mm (Batch 2) using Agilent 1260 UHPLC System

LC Conditions

Column

Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Luna Omega

Part No

00F-4784-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

1.5

80

20

3

16.5

55

45

4

19.5

55

45

5

22.5

80

20

6

25

80

20

Flow Rate

2.00 mL/min

Temperature

30°C

System

Agilent Technologies Agilent Technologies 1260 Infinity

Detection

LC/UV (DAD, PDA) @ 254 nm (nanometers)

Detection Info